Establishment of test conditions and assessment of hormonestimulated growth and druginduced cytostatic and cytotoxic effects. The nci60 cell lines were assembled by the national cancer institute as an in vitro anticancer drug screen, which went into operation in 1990. They both reported 266 and 220 proteins, which is similar to the. The need for in vivo models was an outgrowth of the understanding that cancer cell lines in general and prostate lines in particular do not and cannot recapitulate the disease processes that occur in living tissues. Immortalized benign cell lines bph1 and ep156t, as well as arpositive lncap, lapc4, vcap, ducap. Pc3 pc3 is a human prostate cancer cell line used in prostate cancer research and drug development. The xenografts models nude mice are used for testing the tumorigenicity and metastatic ability of cancer cell lines. Odyssey imager, and analyzed using the provided licor software. We generated rnaseq data from multiple prostate cancer lines and used. Among american men, prostate cancer is the most common, noncutaneous malignancy that accounted for an estimated 241,000 new cases and 34,000 deaths in 2011.
Prostate cancer prostate cancer information and overview. The human prostate cancer cell lines pc3, du145, lncap, 22rv1, and vcap. Common cell lines used in prostate cancer research. Author summary prostate cancer cell lines represent an important model system to characterize molecular alterations that contribute to radioresistance, but irradiation can cause deletions and amplifications of dna segments that affect hundreds of genes. However, its role in mitochondrial metabolism remains unclear. Cosmic, the catalogue of somatic mutations in cancer, is the worlds largest and most comprehensive resource for exploring the impact of somatic mutations in human cancer. Along with lncap and pc3 cells, du145 cells were once considered part of the triad that constituted the gold standard of pca cell culture lines. The gene expression profiles of cell lines showed limited similarities to prostate tumors harvested at surgery. Genomewide association studies identified singlenucleotide polymorphism snp rs55958994 as a significant variant associated with increased susceptibility to prostate cancer. To view a cell lines information, search for it from the front page. Genomic analysis of dna repair genes and androgen signaling in. Using this software, pathway analysis was performed, obtaining.
The ability of the 51gene biomarker to identify chemicals that modulate ar was examined in a compendium of biosets derived from prostate cancer cell lines assembled as described in materials and methods section. Klf5 inhibits angiogenesis in pten deficient prostate cancer. In vitro and in vivo model systems used in prostate cancer research. The dataset comprises four prostate cancer cell lines treated with the dna methyltransferase inhibitor 5azadc. Identification of key pathways and genes in pten mutation.
Prostate cancer is the development of cancer in the prostate, a gland in the male reproductive system. Aug 12, 2003 we did not find mutations in exon 2 of the klf6 gene in any of the 32 tumour samples that we examined nor in the human prostate tumour cell lines pc3, du145 and lncap. Our first job is to listen to and observe what our customers need, and meet those needs with quality products and services. Mouse prostate cancer cell lines established from primary and. An overview of publicly available patientcentered prostate. Screening of a panel of prostate cancer cell lines for parpi sensitivity in order to identify prostate cancer cell lines sensitive to parpi treatment, we performed a screening with a panel of available cell lines cultured under ngc. Using a panel of 64 cell lines to represent various cell populations in different organs and tissues of the human body, the mrna expression of all human genes are characterized by deep rnasequencing. Dna methyltransferase inhibitor 5azadctreated prostate. The second study focuses on identifying radioresistanct biomarkers of prostate cancer. Cell lines immortalized by exposure to human telomerase reverse transcriptase rc58t and rc58thtert. If you have prostate cancer or are close to someone who does, knowing what to expect can help you cope. Tsg101 is a component of the escrt machinery and an established exosomal marker in prostate cancer. Btf3 sustains cancer stemlike phenotype of prostate cancer. Here you can find out all about prostate cancer, including risk factors, symptoms, how it is found, and how it is treated.
Apr 11, 2012 in addition, genistein cooperates with vorinostat to induce cell death in prostate cancer cell lines with a greater effect on early stage prostate cancer. An integrated database of chemosensitivity to 55 anticancer. Atcc stands ready to support our customers needs during the coronavirus pandemic. This in combination with the small number of cell lines that are usually considered does not allow a straightforward identification of. In this study, we show that this variant is located in an enhancer active in prostate cancer cells. The cancer cell types included prostate cancer cell lines lncap, c42, cl1. The urologic epithelial stem cell database uesc a web. In vitro and in vivo model systems used in prostate cancer. Immunohistochemistry and immunofluorescence assays in prostatic tissues. The adca cell lines e2 and e4 and the adica cell lines ce1 and ce2 display biallelic deletion at the pten gene locus, an event which is specific for the prostate epithelium for this mouse model and a fairly similar level of expression of the androgen receptor ar. Wholegenome sequence of the metastatic pc3 and lncap. Atcc normal human primary prostate epithelial cells, when grown in prostate epithelial cell basal media supplemented with prostate epithelial cell growth kit components, provide an ideal cell system to propagate prostate epithelial cells in serumfree conditions.
Proteomic profiling of androgenindependent prostate cancer cell. One way of dealing with that heterogeneity is to test large numbers of patientderived cell lines for common vulnerabilities. Affymetrix array analysis of prostate cancer cell lines. Dec 18, 2018 ncad expression levels transcription and protein were evaluated upon sirna mediated silencing of grp78 in the mm. This cell line was introduced in 1997 and was the result of a series of subcutaneous xenografting experiments into scid mice 55. Behavior of the biomarker in a prostate cancer cell line compendium. These studies examined gene expression profiles in 60 cell lines that were pharmacologically characterized on treatment with various. Cancer cell line encyclopedia novartisbroad, nature 2012 1020 samples. Scientists have established a few prostate cancer cell lines to investigate the mechanism involved in the progression of prostate cancer. Downloading rna seq data for prostate cancer cell lines.
All information is derived from the bc cancer agency prostate cancer cell line database cited previously unless otherwise specified. You did a great service to the cancer research community and by that to the patients that donated the samplesclinical pathologist, karolinska university hospital. The remaining cell lines had an intermediate growth rate. Proteomic profiling of androgenindependent prostate.
This sample is limited in amount and the genetic manipulation is almost impossible 3. The human normal prostate epithelial cell line rwpe1 and human pca cell lines, pc3 and lncap, were purchased from the cell bank of the chinese academy of sciences shanghai, china where they were characterized by dna fingerprinting, isozyme detection, mycoplasma detection and cell vitality detection. The heterogeneity of human cancers is a major obstacle to developing therapies. Olaparib is effective in combination with, and as maintenance. Prostate cancer models cell lines, xenografts, pdx, io models. Properties of common prostate cancer cell lines pc3 pc3 is a human prostate cancer cell line used in prostate cancer research and drug development. Cellminer is a webaccessible relational database tool that organizes, navigates, and integrates multiple types of molecular profile data on the nci60 cell line panel at the t dna, rna, protein, and smallmolecule levels. The ar splice variant ar v567es is constitutively active to investigate the function of ar v567es in both benign and cancer prostate cells, we cotransfected the p69 benign immortalized prostate epithelial cell line and the arnull m12 human prostate cancer cell line, with the ar activity reporter construct pgl3probasin arearr3luciferase arr3luc and a construct expressing ar v567es. Jan 20, 2020 researchers tested approximately 4,518 drug compounds on 578 human cancer cell lines and found nearly 50 that have previously unrecognized anti cancer activity. Identification of androgen receptor modulators in a.
The 1542cptx primary prostate cancer cell line kindly gifted by prof. Find your cell lines and models of interest in one easy search. Kinase pim1 promotes prostate cancer cell growth via cmyc. May 28, 2019 cancer stemlike traits contribute to prostate cancer pca progression and metastasis. Cpdr multicenter national database prostate cancer research. The human prostate cancer cell lines pc3, du145 and 22rv1 were purchased from the american type culture collection atcc and maintained in rpmi1640 medium supplemented with 10% fetal bovine serum fbs, 50 uml penicillin50. In particular the dataset should have patient information such age. Lipogenic signalling modulates prostate cancer cell. The adica cell lines ce series grow well in the absence of androgen.
Hello colleagues, i need to download rna seq data for prostate cancer cell lines like du145 and prec i tried navigating through ccle, oncomine and geo but i cant seem to find rna seq data for normal untreated prostate cancer cell lines. Effects of radiation on prostate cancer cells lines. The prostate cancer risk variant rs55958994 regulates. The panel comprises 60 human cancer cell lines representing nine tissue of origin types. Furthermore, we evaluated the effects of grp78 knockdown kd on epithelialmesenchymal emt transition markers, morphological changes and adhesion of pc3 cells. Two recent proteomic studies revealed exosomal proteins related to prostate cancer. Proteomic profiling of androgenindependent prostate cancer. Jan 20, 2010 the adca cell lines e2 and e4 and the adica cell lines ce1 and ce2 display biallelic deletion at the pten gene locus, an event which is specific for the prostate epithelium for this mouse model and a fairly similar level of expression of the androgen receptor ar. Analysis of the prostate cancer cell line lncap transcriptome. I am looking for a dataset with data gathered from african and african caribbean men while undergoing tests for prostate cancer.
Teasing apart molecular interactions and alterations in cancer cells is of incredible worth, but they do not take into account. The lncap cancer cell line was established from a human lymph node metastatic lesion of prostatic adenocarcinoma. Abstract a database of prostate cancer proteomics has been created by using the results of a proteomic study. Researchers tested approximately 4,518 drug compounds on 578 human cancer cell lines and found nearly 50 that have previously unrecognized anti cancer. Crownbio has made searching for cell lines and xenografts easy using xeno base, our collated online database worlds first online cell line and xenograft. This microarray dataset accompanies the publication by kim et al, the retinoic acid synthesis gene aldh1a2 is a candidate tumor suppressor in prostate cancer.
Rc58t cells were derived from a radical prostatectomy specimen from a 52yearold white man. We present the utility of massively parallel sequencing by synthesis for profiling the transcriptome of a human prostate cancer cell line, lncap, that has been treated with the synthetic androgen, r1881. Sobel re, sadar md 2005 cell lines used in prostate cancer research. The importance of cancer cell lines as in vitro models in cancer methylome analysis and anticancer drugs testing 143 tumour. Cultured cancer cell lines, such as the humanderived pc3 and lncap, are critical for. This dataset provides a context for the use of prostate cancer cell lines as models for clinical cancers. The cancer cells may spread from the prostate to other areas of the body, particularly the bones and lymph nodes. Gene expression omnibus geo database, accession no gse4500. Metastatic castrationsensitive prostate cancer msk, clin cancer res 2020 424 samples. We present the utility of massively parallel sequencing by synthesis for profiling the transcriptome of a human prostate cancer cellline, lncap, that has been treated with the synthetic androgen, r1881. The ubiquitin ligase ube4a inhibits prostate cancer progression by targeting interleukinlike emt inducer ilei. Because du145 and pc3 prostate cancer cell lines are considered to be arnegative,, our observations that. To identify modulators of androgenindependent prostate cancer that could serve as potential biomarkers of aggressive disease, we performed an in depth proteomic analysis of the conditioned media of five androgenindependent prostate cancer cell lines du145, pc3, lncapsf, ppc1, and 22rv1, two androgendependent cell lines lncap and vcap. This project is divided into two distinct studies relative to prostate cancer.
Moreover, prc1 activity correlated with emt and stemness traits in dnpc patient samples and metastatic cell lines, suggesting that prc1 may sustain the oncogenicity of prostate cancer cells that have acquired mesenchymal and stemlike transcriptional traits in support of metastatic capacity. The cell atlas provides highresolution insights into the expression and spatiotemporal distribution of proteins within human cells. In later stages, it can lead to difficulty urinating, blood in. Analysis of human prostate cancers and cell lines for.
The center for prostate disease research cpdr multicenter national database program seeks to longitudinally collect comprehensive demographic, clinical, treatment and quality of life outcomes data to learn more about prostate disease, particularly prostate cancer, in an effort to better prevent, diagnose and treat patients. An overview of publicly available patientcentered prostate cancer datasets prostate cancer pca is the second most common cancer in men, and the second leading cause of death from cancer in men. C silac cell line culture preparation, spikein and mass spectrometry analysis of the proteomes and phosphoproteomes. As and ai cell lines are different in their transcriptional programs and degree of dna copy number alterations. Mutation analysis of 24 known cancer genes in the nci60 cell. Du145 and pc3 human prostate cancer cell lines express. Metabolic rewiring is a common feature of many cancer types, including prostate cancer pca. Our database, comprising both gene expression data and drug activity data for the same set of cell lines, was first developed by ross et al. In the first study, we will determine the cellular response of three prostate cancer cell lines pc3, du145, and 22rv1 after single or fractionated doses of radiation. Characterisation and manipulation of docetaxel resistant. The mutation data table displays a list of mutations specifics to the cell line or gene. For one such study, ras initiative researchers constructed gfplabeled derivatives of dozens of cell lines that express mutant kras. Prostate cancer pca remains one of the leading adult malignancies. Steap2 knockdown reduces the invasive potential of.
Sep 16, 20 fastgrowing cancer cell lines are indicated with a yellow dot and slowergrowing cell lines are indicated by a red dot. Prostate cancer is the most common cancer among men after skin cancer, but it can often be treated successfully. Using appropriate cell lines and cell line derived xenograft models with genetic features of interest can enhance your early stage in vitro and in vivo drug discovery programs. To view a cell line s information, search for it from the front page. Networkbased analysis of prostate cancer cell lines. The alva41 cell line was derived from a bony metastasis from a human prostatic carcinoma by drs.
However, the mechanisms by which this snp mediates increased risk to cancer are still unknown. Alterations in master genes lead to mitochondrial metabolic rewiring and provide an appealing target to inhibit cancer progression and improve survival. Dna copy number changes in prostate cancer cell lines omicx. Pc3 cells are also used to study viral infection in mammalian cells that exhibit an immune response. After 6 passages as monolayers, these cells senesced. Fullyvalidated and characterized prostate cancer in vitro cell lines, in vivo xenografts and pdx for preclinical drug. The student will gather and analyse the masses of genetic data available from prostate cancer patients biopsy and blood samples to work out which treatments, and which clinical trials, might work best for him.
Lncap, pc3, and du145 are commonly used prostate cancer cell lines. So is it good to analyze the prostate cancer cell lines pc3 and du145 or lncap also and then arrive at a. Many studies on pca have been carried out, each taking much time before the data is collected and ready to be analyzed. Neuroendocrine prostate cancer multiinstitute, nat med 2016. Deciphering the novel molecular mechanisms underlying stemlike traits may provide important insight for developing novel therapeutics. Pten phosphatase and tensin homolog mutant is the top common mutated genes in prostate cancer, which makes it a promising biomarker in future individualized treatment. Gpnmboa protein increases the invasiveness of human metastatic prostate cancer cell lines du145 and pc3 through mmp2 and mmp9 activity. We consistently found tsg101 to be present at similar levels in exosomes from both cell lines and thus used this marker for quantification by stripping each respective western blot and reblotting them with a tsg101 specific antibody figure 4a.
B prostate cancer progression over time, from localized asymptomatic castrationsensitive to metastatic castrationresistant disease. In the latest genomewide copy number change studies, klf5s deep deletion, which likely indicates homozygous deletion, has been detected in 10% of prostate cancers in the cancer genome atlas tcga database tcgadata. Epigenetic and genetic features of 24 colon cancer cell lines. Prostate cancer pca is the second most common cancer worldwide, with one in eight men being diagnosed in the uk and one in fivesix in the. The bone metastasisderived pc3 and the lymph node metastasisderived lncap prostate cancer cell lines are widely studied, having been described in thousands of publications over the last four decades. The human prostate cancer cell lines pc3, du145, lncap, 22rv1, and vcap and the normal prostate epithelial cell line rwpe1 were purchased from the american type culture collection atcc, manassas, va. Many specialized cellline databases have been established over the years that cater to a specific taxonomic range or a category of cell lines. Lineage relationship of prostate cancer cell types based on gene.
Adapt identifies an escrt complex composition that. By resequencing the mrna products of a cell, one may rapidly discover polymorphisms and splice variants particular to that cell. Pc3 cells are useful in investigating biochemical changes in advanced prostate cancer cells and in assessing their response to chemotherapeutic agents. Ostenson, american lake veterans affairs medical center, tacoma, washington. Mouse prostate cancer cell lines established from primary. Integrative proteomic and phosphoproteomic profiling of. Name source metastasis in mice doubling time psa rna psa protein ar rna ar protein cytokeratin expression. Networkbased analysis of prostate cancer cell lines reveals novel. The cell line name and lineage are listed at the top of the cell line page. For each cell line, gene expression in treated channel 2.
John masters ucl was grown in ksfm supplemented with 10% fbs, 0. Grp78 modulates cell adhesion markers in prostate cancer and. Start using cosmic by searching for a gene, cancer type, mutation, etc. We obtained gene expression data of prostate cancer from tcga the cancer genome atlas database for analysis.1501 736 1568 1461 15 599 1258 91 575 1288 175 1580 1194 1552 497 1435 846 1240 379 1118 530 194 502 348 841 540 1394 1060 367 719 1047 258 911 733 45 463 1202